Fig 1: Endothelial pro-inflammatory protein secretion in EA.hy926 cells and HUVECs incubated for 24 h with macrophage-conditioned media. THP-1–derived unpolarized, M1-like, and M2-like macrophages were exposed to normoxia (N), chronic hypoxia (chH), or cycling hypoxia (cyH) for 6 h and were then left for 16 h in normoxic air to produce macrophage-conditioned media. Thereafter, EA.hy926 cells (A) and HUVECs (B) were incubated for 24 h with macrophage-conditioned media and the secretion of IL-6, IL-8, and ESM1 in the endothelial cells was assessed by ELISA (n = 3, mean ± 1 SEM). Ctrl corresponds to endothelial cells incubated with CO2-independent medium. Statistical analysis was performed using Student’s t test. *p < 0.05.
Fig 2: Endothelial pro-inflammatory mRNA expression in EA.hy926 cells and HUVECs incubated for 24 h with macrophage-conditioned media. THP-1–derived unpolarized, M1-like, and M2-like macrophages were exposed to normoxia (N), chronic hypoxia (chH), or cycling hypoxia (cyH) for 6 h and were then left for 16 h in normoxic air to produce macrophage-conditioned media. Thereafter, EA.hy926 cells (A) and HUVECs (B) were incubated for 24 h with macrophage-conditioned media and their mRNA expression for IL-6, IL-8, and ESM1 was assessed by RT-qPCR (n = 4, mean ± 1 SEM). Ctrl 1 and Ctrl 2 correspond to endothelial cells incubated with CO2-independent medium or EGM-2 medium, respectively. Statistical analysis was performed using Student’s t test. *p < 0.05; **p < 0.01.
Supplier Page from Abcam for Human ESM1 ELISA Kit (Endocan)